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I have setup postfix to send outgoing mail, It works fine for most of the cases except for some e.g duke.alumni.edu where it gives me a 450 error, even though the email address is valid.

This is the exact error I get Sender address rejected: Domain not found (in reply to RCPT TO command))

Any suggestions on how I can rectify this?

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Just to check, neither a dig to alumni.edu nor to duke.alumni.edu gives any answer. An whois to alumni.edu also returns no match.

You are sure that the subdomain duke.alumni.edu is valid in your environment?

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