Yes, there are many differences between alias and vlan:
VLANs are isolated
When you're using IP alias, you're just propaging new IP on same network. It can be from same subnet or from different one. But anyone who can join to your LAN can access both subnets just adding new IP to their PC. You cannot manage it and secure it. Also anyone can listen to your traffic.
When you're using VLANs, you're creating virtual LANs. Those LANs are strictly separated, node from one VLAN cannot see communication in different VLANs. You can manage MAC filtering on VLAN level, so user cannot join another VLAN just with changing their IP. Also you can propagate VLAN only to some ports, so you can have server VLAN only in server room and IT office without needing of separated cables.
VLANs are just LANs - it can be limited
You can make QoS on your VLANs, different routing rules, firewalls etc. Each VLAN is separated from each other so you can handle it like real LAN.
You need switches and routers which support it
VLAN tag is another header in Ethernet frame, you have to have switches and routers which can reflect it. If you will use switches without support of 802.1q, your separation will not work because those switches will mix it together and send all packets to all.
You can go deeper - q-in-q
You can tagg already tagged frames, so you can have VLAN in VLAN. It is usefull when you want to connect and secure two locality with different ISP. VLAN is normal connection in point of view of PC, so it can looks like those two networks are one big network. This setup needs cooperation with ISP, but works really well.