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Why when I remove the MACHINENAME\Users group permission set from a websites physical folder and and grant a similar permission set to MACHINENAME\IIS_IUSRS group my website will not start.

Why do I have to grant Read & Execute, List folder contents and Read to the MACHINENAME\Users group physical website folder?

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Windows Authentication was enabled on website. Clients who visited the intranet site were granted access via MACHINENAME\Users

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