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Have you ever tried running instances of BizTalk 2006R2 and BizTalk 2009 on the same server?

If yes: were you able to do so without using virtualization?

Any feedback you can provide would be greatly appreciated.

Cheers,

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No, it can't be done. There are too many ways in which BizTalk assumes it's the only BizTalk instance on the machine.

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