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Running Win 2k8 R2 SP1 64 bit as a web server box with IIS 7/.NET 4.

Periodically, I completely lose access to the membership database in one of my sites (ASPNETDB) which causes a "Network Service cannot access Membership DB..." error on the site and makes the whole thing go down! Obviously not good.

Here's what I've already tried (with an Administrator account):

  • GUI tools to take ownership of that file, and of folder above it with propogation
  • Command line tools from an elevated command prompt: takeown, subinacl, icacl
  • Checked with Process Explorer and found no handles to the file

If I reboot the whole server the problem just goes away, but that is obviously not an efficient long term solution. Any other ideas on what/how to troubleshoot?

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And the file is there w/ the expected permissions after the reboot? You're not taking any steps after the reboot to resolve the issue-- it "just works"? – Evan Anderson Aug 23 '11 at 19:31
Yes. There and functioning normally post reboot. – just.another.programmer Aug 23 '11 at 19:31
Same problem here also .. had 4 pdf files that i suddenly couldn't access/change permissions on or take ownership of as Domain Admin. Wft is happening? This is on a Clean installed server W2008 R2 Sp1 Virtualized on Hyper-V. After i rebooted the server everything was ok again. This has happened on other customers servers also. Different AV programs, backup software etc. Only thing common are W2008 R2 and SP1 .. – Nisse Manpower Jan 11 at 10:36
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