We have a number of sites all running essentially the same version of our in house CMS. Each of them is configured as a separate site in IIS7. However on one of them we are getting frequent 404s for URLs that exist. These vary from static files such CSS and JS files to URLs with querystrings in them (eg index.aspx?id-XYZ).

This is only happening on one site. Also it doesn't happen for me :-) or other people with whom I work (note: we all work from home), but our client and their users often sees the site without CSS or download links don't work. As I can see the 404s in the log files, this problem is on our server and not a network issue.

Why would IIS7 return a 404 for a file that is absolutely at the location indicated by the path? Has anyone else had this problem?

Update:

In the log files, I also see sc-substatus is always 0 and sc-win32-status is often 64, but not always. This stands for ERROR_NETNAME_DELETED, but I don't know what that might actually mean.

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