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I had a problem a few days ago see question here: Can no longer access computer or network shares to my server from any other computers on the network

The fix that I found does in fact work however when I boot my PC in the morning the shares are no longer working, to fix this I need to remote desktop into the server and re-install "File and Printer Sharing for Microsoft Networks" on the main network adaptor.

Doing this makes the shares work again, however it is anoying to have to do this each and every morning.

On top of this my offline files are no longer available offline: I store my user profile on the server and had them selected to be "Always Available" however since this has happened they are no longer available offline and the option to make them available offline from the context menu is no longer available.

Another problem, and I don't know if this is the cause or just a tell of a deeper issue but this server runs hyper-v, since these problems I can no longer remote desktop into the hyper-v client.

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You really haven't provided any meaningful details. Read your question from the perspective of someone on the outside and ask yourself if you would even be able to begin to guess. A good start to making this question useful would be to post relevant event log entries and whether or not there are any scheduled jobs that run on the server over night. –  MDMarra Apr 6 '12 at 23:38
    
Users profiles mustn't be available for offline. Windows already keep a copy locally when your log on and push back changes at log off. You must disable offline feature or the roaming profile share. Maybe you just say "user profile" but meant "users documents". Is your server disk accessed through iscsi ? Does the server reboot everynight by itself ? –  Mathieu Chateau Apr 7 '12 at 7:41
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