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Is there a setting in IIS 7.5 to default the "Enable 32-Bit Applications" to False on newly created app pools? I know how to set it for one app pool in power shell, but I'm wondering if there's a global IIS setting for this so it will always be false when a new one is created. Thanks!

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False is the default. Are yours getting set to true then? –  squillman Aug 2 '12 at 20:30
    
Yes, on one of our servers all new app pools are getting set to true by default. –  Dan Aug 2 '12 at 20:37
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The default value for "Enable 32-Bit Applications" is "False" by default. If that is not the case in your setup, you can "fix" it using any of these methods:

Using GUI tool:

IIS Manager | Application Pools | Right click | Set Application Pool Defaults...

Using command line (appcmd.exe in this particular case):

%SystemRoot%\System32\inetsrv\appcmd.exe set config /section:system.applicationHost/applicationPools /applicationPoolDefaults.enable32BitAppOnWin64:"False" /commit:apphost
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That's it, Thanks! –  Dan Aug 2 '12 at 21:04
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