So, every time this issue occurs, I simply open the web.config for the site and save it. This instantly fixes this issue until the next server reboot. This works great, but I do not want to have to do this every time. I saw several posts about this that reference permissions, but that does not seem to be my issue (due to the way I can resolve it each time).
Anyone happen to have some insight as to why this fixes it or how I can fix it permanently?