I set up some GPOs and linked them to the top-level domain in the forest. They had security filtering enabled so that they would apply to a particular group, and everything worked great.
My GPO tree was getting a bit messy so I created a single OU right under Default Domain Policy for these users that need the GPOs. I deleted the previous links and made new ones inside the new OU. But when I run modeling for a test user for a test machine, none of these GPOs are applying anymore.
What changed? Why do the GPOs no longer apply? I thought OUs were not supposed to have any bearing on anything but helping with organization.