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When using anonymous authentication by default IIS uses a specific user named IUSR to access files. This user is automatically a member of the Windows Users but not a member of IIS_IUSRS If you removed permissions for Users from your files, the default site setup wont work anymore. I usually don't use IUSR, to change this in the GUI, select the server or ...


websites with this behavior are Dotnetnuke and I find that it's a problem in dotnetnuke and not related to sql server settings: more details :

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