It very much depends how your cluster is configured and accessed. It doesn't help that teh word "cluster" can cover several different arrangements.
Some arrangements will have very little impact, some will slow things down noticeably (multiple active servers for instance, where a transaction make need to wait for all the servers to be uptodate before it is considered committed which can add considerable transactional latency for operations that include inserts/updates, also marshalling locking information between the servers can affect read-only operations similarly), and some arrangements may speed things up (multiple read-only replicas to farm the read-only load out over multiple machines, locking requirements permitting, for instance).
So: yes, no or maybe! If you describe your arrangement in some detail [machines, OS, DBMS, cluster config, what code accesses the cluster (a single web server, many web servers, many external clients, ...), load patterns, ...] you might get a much better answer.
(so unfortunately it isn't as simple a question as you'd hoped, sorry!)