I have a huge pcap file (generated by tcpdump). When I try to open it in wireshark, the program just gets unresponsive. Is there a way to split a file in set of smaller ones to open them one by one? The traffic captured in a file is generated by two programs on two servers, so I can't split the file using tcpdump 'host' or 'port' filters. I've also tried linux 'split' command :-) but with no luck. Wireshark wouldn't recognize the format.
editcap utility which is distributed with Wireshark.
I know this answer is a little late, but it may serve other people as well. I found a great tool for splitting pcap files: PcapSplitter. It's part of the PcapPlusPlus library which means it's cross-platform (Win32, Linux and Mac OS), and it can split pcap files based on different criteria such as file size (what you seem to need) but also by connection, client/server IP, server port (similar to protocol), packet count, etc. I found it very useful. The link above is for the source code, but if you don't want/know how to compile, I created compiled binaries for several platforms I've been using this tool with. I recommend this tool very much
EDIT: apparently a new version of PcapPlusPlus was released and it contains PcapSplitter binaries for quite a lot of platforms (Windows, Ubuntu 12.04/14.04, Mac OSX Mavericks/Yosemite/El Captian). I think it's better to use these binaries than the link I previously provided. You can find it here
The best and fastest way to go is to use SplitCap, which can split large packet dump files based on sessions for example. This way you'd get each TCP session in a separate PCAP file. SplitCap can also separate packets into pcap files based on IP addresses.
You can read more about SplitCap on the Netresec blog: http://www.netresec.com/?page=Blog&month=2011-05&post=Split-or-filter-your-PCAP-files-with-SplitCap
Download SplitCap from here: http://www.netresec.com/?page=SplitCap
If you're unsure what your PCAP file is really containing, you can visualize it using Squey, isolate packets or sessions worth of interest using arbitrary complex criteria and then export it to smaller PCAP files.