I know that you said you set them up the same, but since they're not behaving the same, and you are asking for help, I'm going to assume that something has been overlooked, so please don't be offended if I mention something that you've already checked:
- One may be using Postscript, while the other may be using PCL
- One may always be sending fonts to the printer every time, while the other is allowing the printer to use fonts stored in memory on the printer
- One may have been set up to use less memory than the other for the print queue
Something that was not clear from your question is whether the two servers are doing the printing, or if a client is printing to the printer via the two servers' print queues. If printing from a client you should remember that while the servers may be configured identically, you may have an obscure setting on your client machine for only one of the printer connections that may be overriding one or more of the default options set on the server. To be sure, you may want to try doing the following on the client machine:
- Delete both printer connections
- Uninstall the printer driver(s) for that printer
- Remove all remaining traces of the driver files from your client machine (including removing the driver from your installation folder(s), e.g. i386, service packs, hotfixes)
- Ensure that the driver files do not return after a reboot (if they have, then the files are probably either subject to Windows File Protection or may be added by a login script, in which case you may want to temporarily disable Windows File Protection, remove the system from the network, drop into Safe Mode without networking, etc. until you have completely banished the driver from the system, even after a reboot, with the system connected to the network)
- Add both printer connections from the respective servers using the same method
If printing directly from the two servers, you may want to also check to ensure that the software doing the printing (e.g. Word, Acrobat, etc.) is exactly the same version and also configured exactly the same on the two servers.