How can i take a complete backup of windows Active Directory into a single file and is it possible to restore this AD backup to another server?
I cannot use active directory replication now, because of the connectivity issues.
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You should always have more than one domain controller to help avoid this!
Microsoft provides documentation for this task. For recent versions, you can use the windows server backup tool to perform an unscheduled backup and then later a full restore: https://technet.microsoft.com/en-us/library/cc753359(v=ws.10).aspx
You will want to read through the dozens of notes and cautions before you start. I would also check DNS very carefully after.