1

A GPO was setup with filtering applied to a single group containing computer objects. Is a restart mandatory for all the computer objects listed in the "New Group" for GPO to be applied on them? or is there other way without restart?

2

Do you mean that you just added the computer accounts to the Security Group you're using in your GPO filter? If so, then the computers need to be rebooted.

1

No, while there are ways to get a new Kerberos token with refreshed group membership, a reboot is required for everything to take effect.

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