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I have a 2000 member server in a 2003 FFL/DFL domain with a 2003 DC. I'm hoping to stand up a new 2012R2 DC, transfer fsmo roles and demote the 2003DC.

If I keep the FFL/DFL at 2003, will the 2000 member server still be able to authenticate once I only have 2012 DCs?

closed as off-topic by Greg Askew, kasperd, mdpc, Diamant, HBruijn Feb 5 '16 at 13:58

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Every Windows O.S. starting with Windows 2000 will be able to authenticate against any Active Directory domain controller; you can only have issues if you still have NT 4.0 systems lingering around, because they only support old-style NTLM authentication which is not in use anymore on recent DCs.

That said, you really shouldn't keep Windows 2000 systems around, if at all possible.

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